Using the Sperm Quality Analyzer (SQA IIc) to evaluate dog ejaculates

نویسنده

  • P. PRINOSILOVA
چکیده

38 fresh ejaculates were examined, of which 23 were cryopreserved in three diluents differing in concentration of glycerol and examined after thawing. For each ejaculate we established the concentration, motility, speed of sperm, percentage of live sperm and the morphological image of the ejaculate using routine laboratory diagnosis and SQA parameters at the initial value and after a 120-minute survival test. The values of the parameters found through routine diagnosis and using the SQA device were statistically confronted. From the results it emerges that the device functions on the basis of the turbulence effect of the semen, and therefore on the intensity and character of the moving mass of sperm. From a research perspective, the SQA device is less usable, since the values it provides are not absolutely comparable with the values found through routine laboratory diagnosis. For the practitioner, however, the device may be useful, since the value of the sperm motility index (SMI) indicates the quality of the semen through the close connection of two parameters, concentration and motility. For a more precise assessment of the ejaculate, however, it is necessary to also analyse the remaining parameters of routine laboratory sperm analysis. To be useful in clinical practice for the evaluation of semen, it would be necessary to establish SMI limit values for ejaculates of varying quality.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Importance of papanicolaou staining for sperm morphologic analysis: comparison with an automated sperm quality analyzer.

Without experience or proper training, the evaluation of the morphologic features of sperm can become the most confusing and time-consuming area of semen analysis. This study defined the role of Papanicolaou staining compared with an automated sperm analyzer in the management of infertility. We compared the morphologic features of sperm using Papanicolaou staining and an automated Sperm Quality...

متن کامل

Automation is the key to standardized semen analysis using the automated SQA-V sperm quality analyzer.

OBJECTIVE To evaluate the performance of the automated semen quality analyzer system for assessing sperm quality. DESIGN Double-blind prospective study. SETTING Tertiary care hospital. PATIENT(S) Fifty healthy men donated semen samples. INTERVENTION(S) None. MAIN OUTCOME MEASURE(S) Precision, accuracy and agreement between automated and manual semen analysis methods was assessed for s...

متن کامل

Influence of the Prostatic Fluid from the First and Third Fractions of the Ejaculates on the Cryosurvival of Poodle Dog Sperm

Problem statement: Two experiments were conducted to determine the effect of the prostatic fluid from the first and third fractions of the ejaculates on the freezability of poodle dog sperm. Approach: The ejaculates from 2 trained poodle dogs were collected. Effect of prostatic fluid from the first and third fractions of ejaculates on cryosurvival of dog sperm was examined. SDS-PAGE analyses we...

متن کامل

Sperm motility index and it’s relation to sperm concentration in subjects with impaired fertility potential

A cross sectional study of sperm motility index (SMI) in normozoospermic and oligoasthenoteratozoospermic males was undertaken to find correlation between sperm concentration and SMI. Two-hundred subjects with impaired fertility potential were screened for the routine seminogram parameters along with SMI using sperm quality analyzer (SQA IIB) to find out the SMI in the population of central Ind...

متن کامل

Factors influencing boar sperm cryosurvival.

Optimal sperm cryopreservation is a prerequisite for the sustainable commercial application of frozen-thawed boar semen for AI. Three experiments were performed to identify factors influencing variability of postthaw sperm survival among 464 boar ejaculates. Sperm-rich ejaculate fractions were cryopre-served using a standard freezing-thawing procedure for 0.5-mL plastic straws and computer-cont...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2004